Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat-responsive proteins from total endosperm.
Identifieur interne : 000009 ( Main/Exploration ); précédent : 000008; suivant : 000010Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat-responsive proteins from total endosperm.
Auteurs : Thouraya Majoul [Tunisie] ; Emmanuelle Bancel ; Eugène Triboï ; Jeannette Ben Hamida ; Gérard BranlardSource :
- Proteomics [ 1615-9853 ] ; 2003.
Descripteurs français
- KwdFr :
- Bases de données comme sujet (MeSH), Chromatographie en phase liquide à haute performance (MeSH), Concentration en ions d'hydrogène (MeSH), Protéome (MeSH), Spectrométrie de masse MALDI (MeSH), Température (MeSH), Température élevée (MeSH), Triticum (métabolisme), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (MeSH), Databases as Topic (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Hot Temperature (MeSH), Hydrogen-Ion Concentration (MeSH), Proteome (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Temperature (MeSH), Triticum (metabolism).
- MESH :
Abstract
High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to determine the influence of high temperature during grain filling on the protein composition of bread wheat evaluated by proteomic tools. Plants were grown in the field and transferred to cabinets soon after flowering. They were subjected to two thermal regimes 18 degrees C/10 degrees C (day/night) and 34 degrees C/10 degrees C. Total proteins were extracted from control grains and treated plants at three different post-anthesis stages. The proteins were separated by two-dimensional gel electrophoresis and analysed by Melanie 3 software. Of the total number of mature wheat grain proteins, 37 were identified as significantly changed by heat treatment. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the characterization of 25 heat-induced proteins and only one heat-decreased protein spot. To learn more about the function of the identified proteins, we examined their expression during treatment.
DOI: 10.1002/pmic.200390026
PubMed: 12601810
Affiliations:
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Le document en format XML
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type="abstract" xml:lang="en">High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to determine the influence of high temperature during grain filling on the protein composition of bread wheat evaluated by proteomic tools. Plants were grown in the field and transferred to cabinets soon after flowering. They were subjected to two thermal regimes 18 degrees C/10 degrees C (day/night) and 34 degrees C/10 degrees C. Total proteins were extracted from control grains and treated plants at three different post-anthesis stages. The proteins were separated by two-dimensional gel electrophoresis and analysed by Melanie 3 software. Of the total number of mature wheat grain proteins, 37 were identified as significantly changed by heat treatment. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the characterization of 25 heat-induced proteins and only one heat-decreased protein spot. To learn more about the function of the identified proteins, we examined their expression during treatment.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country><region><li>Gouvernorat de Tunis</li></region><settlement><li>Tunis</li></settlement></list><tree><noCountry><name sortKey="Bancel, Emmanuelle" sort="Bancel, Emmanuelle" uniqKey="Bancel E" first="Emmanuelle" last="Bancel">Emmanuelle Bancel</name><name sortKey="Ben Hamida, Jeannette" sort="Ben Hamida, Jeannette" uniqKey="Ben Hamida J" first="Jeannette" last="Ben Hamida">Jeannette Ben Hamida</name><name sortKey="Branlard, Gerard" sort="Branlard, Gerard" uniqKey="Branlard G" first="Gérard" last="Branlard">Gérard Branlard</name><name sortKey="Triboi, Eugene" sort="Triboi, Eugene" uniqKey="Triboi E" first="Eugène" last="Triboï">Eugène Triboï</name></noCountry><country name="Tunisie"><region name="Gouvernorat de Tunis"><name sortKey="Majoul, Thouraya" sort="Majoul, Thouraya" uniqKey="Majoul T" first="Thouraya" last="Majoul">Thouraya Majoul</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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